Madam Mim is the main antagonist of the 1963 animated film The Sword in the Stone, and of the Descendants novel, Return to the Isle of the Lost: A Descendants Novel. She has several granddaughters, among them Mad Maddy.

The Stabbington brothers are the secondary antagonists of the 2010 animated film Tangled, where after stealing the royal crown and being betrayed by Flynn Rider they allied themselves with Mother Gothel to ambush Rapunzel and Rider in order to take revenge on the latter, with which they were sent to the Isle of the Lost.They are only referenced as the fathers of the Stabbington cousins.

One of The Stabbington brothers are the secondary antagonists of the 2010 animated film Tangled, where after stealing the royal crown and being betrayed by Flynn Rider they allied themselves with Mother Gothel to ambush Rapunzel and Rider in order to take revenge on the latter, with which they were sent to the Isle of the Lost. They are only referenced as the fathers of the Stabbington cousins.

Iago is one of the henchmen to be imprisoned in the Isle of the Lost. He is Jafar's sidekick in the animated film Aladdin. He is now the father of some unnamed parrots and one of them is named Othello.

Horace Badun is the secondary antagonists of the 1961 animated film 101 Dalmatians, where he helped Cruella De Vil in her plans to kidnap Dalmatian puppies to make a coat with their furs, with which he were sent to the Isle of the Lost. he is only referenced as the father of Harry Badun.

Jasper Badun is the secondary antagonists of the 1961 animated film 101 Dalmatians, where he helped Cruella De Vil in her plans to kidnap Dalmatian puppies to make a coat with their furs, with which he were sent to the Isle of the Lost.he are only referenced as the father of Jace Badun.

The Ringmaster is an antagonist of the 1941 animated film Dumbo, where he locked up Mrs. Jumbo, and made her son act as a clown after ruining the show with which he hoped to succeed, with which he was sent to the Isle of the Lost.He is the father of Hermie Bing. He is referenced in the novel Return to the Isle of the Lost: A Descendants Novel, where his daughter is introduced.

Governor Ratcliffe is the main antagonist of the 1995 animated film Pocahontas, where he invaded the New World in search of gold, without caring about killing its inhabitants to get it, for which he was sent to the Isle of the Lost.He is the father of Rick Ratcliffe. He is referenced in the novel The Isle of the Lost, where his son Rick is introduced.[7] He is mentioned in the book Descendants: Mal's Spell Book, where Mal has him listed among other villains as "Those Entitled to Respect".

Edgar Balthazar is the main antagonist of the 1971 animated film The Aristocats, where he kidnapped Madame Bonfamille's cats to be the sole heir to her fortune, for which he was sent to the Isle of the Lost.He is the father of Eddie Balthazar. He is referenced in the novel Return to the Isle of the Lost, where his son Eddie is introduced, being mentioned the time when he was intent on drowning Duchess and her kittens.[5] He is also mentioned in the book Descendants 3: The Villain Kids' Guide for New VKs.

Banzai is the secondary antagonists of the 1994 animated film The Lion King, where he helped Scar in his plans to take over the throne of the Pride Lands, for which he were sent to the Isle of the Lost. He is mentioned in the novel The Isle of the Lost as the parents of some Spotted Hyenas.

Judge Claude Frollo is one of the villains imprisoned in The Isle of the Lost, being the main antagonist of the 1996 animated film The Hunchback of Notre Dame. He has a daughter named Claudine Frollo who is the bell ringer of Dragon Hall. He is also the owner of Frollo's Creperie and former host of Judge Frollo, a TV show that aired on Evil Isle.

Clayton is the main antagonist of the 1999 animated film Tarzan, where he tried to hunt Tarzan's gorilla family, with which he was sent to the Isle of the Lost.He is the father of Clay Clayton. He is referenced in the book The Isle of the Lost, where his son Clay is introduced.[4]

Yzma is the main antagonist of the 2000 film The Emperor's New Groove, where she tried to take down Emperor Kuzco to usurp his throne, with which she was sent to the Isle of the Lost. She is the mother of Yzla and Zevon.