EVE is a member of the 7th Airborne Squad as part of the Eve Defense Force and the main playable protagonist of Stellar Blade. Originating from the off-world Colony where the human race shelters, EVE's arrival on Earth is a disaster. As the 7th Airborne Squad begins its operation to reclaim the planet from the Naytibas, a ferocious surprise attack leaves her unit decimated. With the odds firmly against her survival, EVE is saved by a scavenger named Adam, who guides her into Xion, the last surviving city on Earth. Aided by Adam – and later an engineer named Lily – EVE vows to continue her mission: the complete elimination of the Naytibas.

Noir is a member of 777, the squad that runs the Ark's casino resort Coin Rush. Despite being the older sister, she's shy and timid compared to Blanc.

A Nikke who is part of Wardress, the Rapture Capture Squad. Don't let her innocent appearance fool you, she's enjoys pain and gets a kicks from it. She even has empathy for her abusers, and attempts to rationalize the behavior of those who mistreat her. She is a close friend with her squadmate Yuni.

A master escape artist who can break out of any prison where she is being kept captive. A highly amiable individual who excels in wheeling and dealing. She is always the life of the party at the Rehabilitation Center. She has a knack for making connections, placing her squarely at the epicenter of the Rehabilitation Center’s economy.

A member of the Central Government Triangle squad. Notorious for treating others poorly, she is regarded as a princess and considers Rapi her rival.

Red Hood is a member of the ill-fated Goddess squad led by the Legendary Commander consisting of her, Liliweiss, Dorothy, Rapunzel, Scarlet, and Snow White. Her notable characteristics are her fondness in both country music and adult magazines. She was formerly assimilated with Rapi. The last Grimms model joining the Goddess squad. She's a bold and cheerful Nikke who loves old songs.

A talented doctor who treats her patients with an unsurpassed solicitude. Mary works as a doctor in the medical squad Seraphim alongside Pepper. Mary is known as the "White Angel" for her gentle demeanor, but for those patients who misbehave, she's feared as the "Black Devil", brandishing a shiny metallic syringe full of sedative.

Elegg is a Nikke from Electric Shock, an R&D squad specializing in electronics. Her favorite hobby is playing BOOM, a first-person shooter video game.

An endearing individual who often indulges in fantasy with a somewhat unique worldview. She's a member of Unlimited, and spends her days looking for Rabbity. Alice has a wild imagination and views the entire world as a kind of sprawling fairytale kingdom.

A Nikke subordinate of the Unlimited Squad, she is always searching for polar bears. Has a proclivity for hibernating, as she feels imitating the behavior of polar bears is the key to finding them.

A Nikke subordinate of the Real Kindness Squad. Possesses incredible strength. Detained in the Rehabilitation Center for causing severe bodily damage to over 40 Nikkes within 2 weeks. She runs the Rehabilitation Center and has strength far superior to other Nikkes. Unable to control her own power, she is locked up currently.

Leader of Absolute, a miracle squad that has never lost. Possessing immense firepower, charisma & splendid troop management, making it hard to manage her. Due to her intense perfectionism, Eunhwa speaks in a cold, aloof, and rather brash manner, especially to strangers. Her extreme judgment and criticism towards other Nikkes usually led to her being disliked.

M.M.R. Vocational School teacher. Her belly ring is a special device which helps make informed decisions in an emergency. Has a drone as a secretary. The rational, perfectionist teacher at M.M.R. Vocational School. She continuously strives to provide excellent education to students. She also has a girly side and adores cute things.

Diesel is a member of InfinityRail, a NIKKE squad that works on the Ark Express. Easygoing, always smiling, never gets mad at others.

Something of a sister figure for Absolute, who enjoys caring for others. Yet, there are times when she lets her guard down.

Anis is a Nikke from Tetra Line. She uses her rocket launcher "Liberty's Tail" as her weapon. A girl that knows exactly how to survive in this forlorn world. Somewhere in between somber Rapi and off-the-wall Neon, she is fond of telling jokes to lighten the mood whenever things get too tense.

Rapi is a Nikke manufactured by Elysion and the leader of the Commander's squad, Counters, as well as the deuteragonist of the campaign. She uses an Assault Rifle called "Militaria" as her weapon. A troubled yet reliable girl who seeks to answer the question of just what Nikkes really are. As a member of Counters, she always ensures harmonious relations between various squadmates.