Resident Uniqua is a citizen of Mega City. She owns a robot named Reba who has broken down due to one of Professor Bug's mechanical bugs. Resident Uniqua is mostly pink. She wears gray overalls with white spots. Her clothes are nearly identical to Uniqua's standard outfit, aside from the color scheme. Uniqua has played Resident Uniqua in two episodes of The Backyardigans: "Robot Rampage: Part 1" and "Robot Rampage: Part 2" from season four.

The little girl is an explorer in a storybook that Austin reads. She loves snow and wonders what causes snow to fall. To find the secret of snow, she visits Ice Lady at her factory. The Ice Lady considers her an interruption and sends her away to far-away locations. She befriends Cowboy Pablo, Tyrone of the Jungle, and Assistant Austin on her journey. When she finally asks the Ice Lady about the secret of snow, she discovers that it does not exist and learns that friends are more important than snow. The Little Girl is mostly pink. She wears a light blue vest, a purple tuque with a white pompom, a matching scarf, and mittens. Uniqua has played the little girl in one episode of The Backyardigans: "The Secret of Snow" from season two.

Ninja Uniqua is a martial artist with a fondness for pie. She is the partner of Ninja Pablo. Ninja Uniqua is mostly pink. She wears a black shozoku and a magenta mask. Uniqua has played Ninja Uniqua in one episode of The Backyardigans: "Samurai Pie" from season two.

Kiara is the daughter of Simba and Nala, Kion's older sister, the mate of Kovu, the grandniece of Scar, the granddaughter of Mufasa, Sarabi, Sarafina, and Nala's Father, and the future Queen of the Pride Lands. She is a major character in The Lion Guard: Return of the Roar and a recurring character in The Lion Guard. As a teenager and a young adult, Kiara doesn't change much from a pre-adolescent except that she has gotten bigger in size and her jaw is slightly wider in comparison to her face. Kiara is kind, loyal, strong, determined and independent. She is assertive and sometimes stubborn in her decisions. She's also a little snarky towards her younger brother, who is equally snarky back, though she truly does care for him. Kiara often employs sarcasm, when she is irritated. However, she is also polite and doesn't hesitate to express her gratitude. Kiara shows much excitement in becoming the future queen of the Pride Lands. She displays a great sense of responsibility, especially in her position as the future queen of Pride Rock, despite having reservations about the lack of fun and freedom when she was a very small cub. She also respects and understands the Circle of Life, and willingly shares her knowledge of it with her friends. Kiara loves and cares for her father Simba and does not want to let him down. Bad odors bother her, though she did not mind when Bunga used them to save her. Unlike her friends Tiifu and Zuri, Kiara believes that there are more important things than appearance She also have saved them when they are in danger. Despite being friends with the two, she doesn't always approve of their antics and can either get frustrated or call them out for their behavior.

Miss Rhamaswami is a wealthy Indian lady played by Uniqua. She is greedy, but also playfully innocent and naïve, having previously wanted an Asian elephant as a pet. After meeting Park Ranger Tasha, she changes her mind and decides to keep the elephant at the Dandeli Wildlife Sanctuary. Miss Rhamaswami owns a private jet and works with her devoted assistant Austin-Ji. Miss Rhamaswami is mostly light pink, with darker pink spots. She wears a purple shalwar and kameez with gold embroidery. She also wears a purple turban with a feather. Uniqua has only played as Miss Rhamaswami in one episode of The Backyardigans: "Elephant on the Run" from season four.

Boots is a five (four for the first 12 episodes) year old anthropomorphic monkey and Dora's best friend who is the deuteragonist and co-host of the series. He likes to hold Dora's hand. Boots sometimes gets discouraged when things go wrong. When he is feeling down, he always bounces back quickly with one of his flips. No one can make Dora laugh the way Boots can. Boots is a character who Dora met one day in "Dora's First Trip", and is her best friend. He is friendly and enthusiastic and usually, wears his beloved red boots, hence his name. His parents look similar, with variations in eyes, height, and clothing. Boots is present with Dora on most of her adventures, and he helps Dora to solve clues and puzzles. He also loves baseball and is on a team with Dora. He loves to ride Rojo the fire truck and Azul the train. Boots is an athletic and energetic monkey. He performs a series of acrobatic flips, somersaults, and cartwheels, along the way to their destination. His lightweight also makes him easy to carry, even for Dora. Boots also "loves" many things. In one episode, he would say many times (e.g. "I love riddles. Call me 'Mr. Riddles'"; "I love nuts"; "I love chocolate"; "I love my ball"; "…and I love, love, love..."). Most importantly, however, Boots loves Dora and Dora loves him back, as both expressed verbally and physically in numerous episodes. His devotion and love always has Boots more than willing to drop whatever he's doing at the moment and make space in his random mental schedule for Dora. For her sake, he has even engaged in his own single adventures assisted by the viewers. Boots' Spanish vocabulary is limited but steadily increasing. Dora teaches him Spanish through "memorable" songs and phrases.

Izzy is one of the two deuteragonists in the Disney Junior animated series, Jake and the Never Land Pirates. She is a feisty young pirate girl and a member of Jake's pirate crew. She was originally voiced by Madison Pettis and later by Megan Richie. Izzy is a feisty pirate young pirate girl who has the ability to fly due to her Pixie Dust given to her by Peter Pan and the fairies. She is beautiful and just as adventurous as Jake and Cubby. She loves puzzles, music, and of course singing and dancing. She seems to be the brightest member of the trio. She treats animals like her own children and cares for them. She is also part of Jake's crew on their pirate ship Bucky. Izzy treats Cubby like a sibling and cares for him deeply. She thinks of Jake as an older brother and more than a friend at the same time. Izzy shares some traits with Tinker Bell most notably her feisty attitude. Izzy believes that with only practice will you achieve a goal showing that she's a hardworking and strong willed pirate. Izzy shows little fear of Captain Hook but does not let it get in the way of her and her friends saving the day. She is not afraid to stand up to Hook and shows her anger when he betrays them and steals from them. Izzy and Mr. Smee rarely communicate but they seem to be more acquaintance like than enemies. Smee is just as kind to Izzy as he is to Jake and Cubby.

Map (sometimes referred after "The") is a main character on Dora the Explorer. He is Dora's helpful guide who lives inside Backpack's side pocket. Described as a "funny, bouncy, rolled up map", Map is a kind and smart character who often helps Dora through her adventures by showing her where to go via his map. He is often a very useful friend who helps the audience of kids know where the blank is coming from. He also loves and performs rap. Unlike Backpack, who's shown to speak English and Spanish frequently, Map is often shown to use English more, though he's not incapable of using Spanish, such as in Super Map, where he helps Tico down the River by following either the red / rojo, or yellow / amarillo paths. Map has a rather simplistic design and color pallet. Since his character design is based around paper, he has two sides: his outside is a pale-yellow, partially beige, and his inside is light blue with his eyebrows being the same color. His mouth is red, and he has two white eyes with black pupils. In seasons 7 and 8, he becomes a computer-generated character, just like Backpack and the Fiesta Trio.

Backpack (or Mochila in Spanish) is a friend and special helper to Dora who helps her on all of Dora's adventures. Just like Dora, Backpack can speak both Spanish and English. Backpack is a real go-getter with a can-do spirit. As Backpack says in her signature song, "Anything that you might need, I've got inside for you." From binoculars to sticky tape, Backpack's loaded up with everything Dora might need for her quest, though it is up to the viewer to pick out the item(s) that will solve the problem at hand. Just like both Maps, she opens her own segment (the helping segment, known as the backpack segment). They also live in her side pocket. Backpack is a purple flap backpack, a type of backpack that opens into one large main pocket with a flap accessible at the top. Throughout Dora the Explorer, Backpack consisted of two purples: a lighter purple for her flap, face, and straps, and a darker purple for all the other sides and her two side pockets, either of which houses The Map. Her flap is also shown to be able to hide her face which has two circular white eyes with black pupils, all underneath two yellow eyebrows. The inside of her mouth is colored burgundy.

Sir Garrett, normally called Garrett, is one of the main characters on Nella the Princess Knight. Garrett is another knight in the kingdom of Castlehaven. He has a steed named Clod and often joins Nella on her adventures. He collects trading cards called Dragon Cards that identify different kinds of magical creatures.

Tasha is a yellow hippopotamus and a major character in The Backyardigans. Tasha's speaking voice was provided by Naelee Rae in the first two seasons and by Gianna Bruzzese in the final two seasons. Her singing voice was initially provided by Kristin Klabunde, with Gabriella Malek taking over during the third season and continuing to voice the character for the remainder of the series. Tasha is assertive and likes to be in charge most of the time. She is rational, skeptical, and highly motivated to get her own way. Tasha is normally very opinionated. She is difficult to frighten, a fact she takes pride in. Tasha tends to play a somewhat antagonistic role throughout the first season, often acting bossy towards the other characters. In later episodes, her personality becomes bolder and less overbearing. Tasha lives in an yellow-colored house to the left of Pablo's home. It is visited the least out of the five main characters' residences. Whenever the Backyardigans are shown heading to Tasha's house for a snack, they walk behind Pablo's house to get there. Tasha is a yellow-skinned hippo with a red-pink tongue and two nostrils above her mouth. She wears a pair of red Mary-Janes and an orange dress with eight light-orange flowers printed on it in random spots. Tasha appears in many episodes of The Backyardigans. Her first appearance was in "Me and My Friends", the unaired live-action pilot. Her first appearance in an aired episode was in "The Yeti". Her last appearance in an episode of The Backyardigans was in "The Tale of the Not-So-Nice Dragon". Her last appearance in any aired animation was in Nickelodeon's Mega Music Fest 2010.

Alicia Márquez is the deuteragonist of the series and is Diego's older sister. She loves to help animals as much as her brother does. She would sometimes join Diego and Dora on their rescue missions out in the field. Alicia is Diego's slightly tomboyish 11-year-old sister and resident computer whiz. She adores her little brother and is amazed at his natural gift for communicating and bonding with animals. Alicia is also protective of Diego and warns him of approaching dangers via his video watch from her monitoring position at the Rescue Center. She can also find any information and facts on animals on the Rescue Computer to help Diego start his mission. Alicia appears to have a peach-brown skin color. Her hair being shoulder length. In Seasons 1-3, she appears to be wearing a red shirt, light green shorts, a tan vest, a red and yellow bracelet, yellow socks, and light gray and white shoes. Her ears are pierced with blue pearls. Alicia is the middle child of Nico and Sabrina, the younger sister of Daisy, and the second eldest sister of Diego. Her cousins are Dora, Guillermo, and Isabella. Her aunt and uncle are Elena and Cole Márquez.

Alicia Márquez is the deuteragonist of the series and is Diego's older sister. She loves to help animals as much as her brother does. She would sometimes join Diego and Dora on their rescue missions out in the field. Alicia is Diego's slightly tomboyish 11-year-old sister and resident computer whiz. She adores her little brother and is amazed at his natural gift for communicating and bonding with animals. Alicia is also protective of Diego and warns him of approaching dangers via his video watch from her monitoring position at the Rescue Center. She can also find any information and facts on animals on the Rescue Computer to help Diego start his mission. Alicia appears to have a peach-brown skin color. Her hair being shoulder length. In Seasons 1-3, she appears to be wearing a red shirt, light green shorts, a tan vest, a red and yellow bracelet, yellow socks, and light gray and white shoes. Her ears are pierced with blue pearls. Alicia is the middle child of Nico and Sabrina, the younger sister of Daisy, and the second eldest sister of Diego. Her cousins are Dora, Guillermo, and Isabella. Her aunt and uncle are Elena and Cole Márquez.

Daisy Márquez is the big sister of both Alicia and Diego, and the eldest cousin of Dora, Guillermo and Isabella. She appears in some episodes of Dora the Explorer and Go, Diego, Go!, but has more appearances in the former series. In the October 2004 (or produced 2003) episode Daisy, La Quinceañera, she turns 15. This would make her 14 in her first appearance in the November 2003 (or produced 2002) episode Dora Saves the Game. At some point during Go, Diego, Go! (which ran from 2005 to 2011), Diego stated that Daisy was in college. Depending on when this occurred, it may be a hint that she’s a prodigy or it was referring to college in its definition outside of the United States (meaning High School). Dora and Daisy both like playing soccer. Her soccer team was the Yellow Tigres or the yellow tigers. In Dora Saves the Game, Dora joined the Yellow Tigres because her cousin's soccer team didn't reach the maximum amount of players. Daisy has tanned skin and dark brown eyes. Her hair is very a dark shade of brown, as well. Her hair is about two or three inches above the mid of her shoulders. Daisy is taller than her younger cousin Dora, younger sibling Alicia, and youngest sibling Diego. She has more tanned skin and hair than her younger siblings. Her eyes are brown just like Dora's eyes. Her hair is dark brown with a barrette in it. As a teenager, she is one of the few known characters to take personal care of herself.

Allie is a girl who lives in the Crystal Kingdom. She first appeared in the double-length episode Dora Saves the Crystal Kingdom. She also appeared in a flashback of said episode in Dora's Big Birthday Adventure. Allie wears a red dress with yellow, dark blue, and aqua blue stripes. As queen, she wears the King's crown as well as a purple dress with the sleeves being pink with magenta stripes. She has blue eyes, and blonde hair. Allie met Dora and Boots when she saw a crystal shining light into the storybook. She comes out of the book. Soon after, Allie realized that the crystal Dora had around her neck wasn't one of her crystals and Allie was in desperate need of help. The crystal kingdom has lost its color because the greedy king hid 3 crystals in 3 different stories and kept the red crystal very closely to his side. So, Dora and Boots accepted the request to help Allie search for the 4 crystals in 4 different stories. Along the way, Dora, Boots & Allie teach the greedy king that it's important to share. The Greedy King then gave Allie his crown, and was crowned Queen of the Crystal Kingdom. Her people even chanted "Allie es la Reina!". She later appears in the special Dora's Big Birthday Adventure, both in flashbacks and near the end when she (along with Sabrina the Snow Princess) wishes Dora back home for her birthday party.

Mariana the Mermaid is a girl who was first introduced in the double-length episode "Dora Saves the Mermaids". She is voiced by Gabriela Aisenberg in Dora the Explorer and Go, Diego, Go!, and by Naomi Rosado in Dora and Friends: Into the City!. She is described as a beautiful mermaid. Mariana has strawberry-blonde hair with a flower shaped like a star in her hair. She wears a purple mermaid shirt and her fish tail (Manatee tail in Manatee's Mermaid Rescue!) is cyan.

Sabrina the Snow Princess (also known as "Princess Sabrina", "Snow Princess", or even "La Princesa") is a girl who appeared on Dora the Explorer episodes which took place in the Snowy Forest. She first appeared in Dora Saves the Snow Princess. Before Sabrina became a snow princess, she had lived in a forest where it never snows. After she freed a white dove (who would later be a snow fairy) from a trap, it flew to a bush with purple berries and underneath was a magic crystal. Sabrina pulled out the crystal and smiled into it, transforming her into the snow princess while the white dove turned into the Snow Fairy. This made lots of icy blizzards to make the forest filled with snow. She wears a light blue princess dress with two purple stripes with snowflakes on it, a snowflake crown, matching snowflake earrings and a pearl necklace, and white slippers. She has brown hair and gray-green eyes. Before she became a princess, she wore a yellow, blue, and purple shirt with blue pants and white shoes.

Daisy Márquez is Diego and Alicia's older sister. She almost never appeared in the show. She appeared in "The Bobo's Mother's Day" with her little brother and sister stating that she's home from college even though she's only fifteen (as she celebrated her fifteenth birthday previously in Dora the Explorer). She's possibly in college because she's a prodigy (of course it is possible she could be older in Go, Diego, Go!). Daisy is also seen in "Rainforest Rhapsody" in the audience while with Dora and the Bobos watching her sister's concert. Since Daisy hardly ever appears in Go, Diego Go!, not much is known about her.

N'Dari is a boy from Tanzania. His only appearance was on Dora's World Adventure from the TV show Dora the Explorer.

Mei is a Chinese girl who was first introduced in the double-length episode Dora's World Adventure.